Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Research Products International Corp MES Monohydrate 5 KG
Buffer for use in plant tissue culture.
- pKa (20°C): 6.15
- pH range: 5.5 - 6.7
Research Products International Corp TRIS, Buffered Saline, 500 ML
TBS 10X Solution (Tris Buffered Saline) is a concentrated buffered salt solution containing Tris and sodium chloride. It maintains pH stability and provides ionic strength for biological experiments. Tris acts as a buffering agent to keep pH consistent, while sodium chloride mimics physiological conditions. Dilute 10X solution with distilled water to prepare working concentrations (e.g., 1X). TBS is widely used in protein and nucleic acid blotting, immunohistochemistry, and molecular biology techniques, ensuring stable and reproducible results across diverse applications.
Sigma Aldrich Fine Chemicals Biosciences HEPES anhydrous, free-flowing, Redi-Dri(TM), >=99.5% | 7365-45-9 | MFCD00006158 | 1KG
HEPES anhydrous, free-flowing, Redi-Dri(TM), >=99.5% | Purity: >=99.5% | Mol Wt: 238.3 | 7365-45-9 | MFCD00006158 | 1KG
Research Products International Corp ADA 100 G
- Substance Name: [N-(Carbamoylmethyl) iminodiacetic acid)] [N-(2-Aceta-mido)iminodiacetic acid]
- CAS Number: 26239-55-4
- Molecular Formula: C6H10N2O5
- Molecular Weight: 190.2
Research Products International Corp TAE, 10 L
TAE 10X Solution is a concentrated Tris-acetate-EDTA buffer used in molecular biology for DNA and RNA electrophoresis. When diluted to 1X, it maintains pH (~8.3) and low ionic strength. It supports clear nucleic acid separation, extended run times, and protects samples from degradation by chelating divalent metal ions like Mg²⁺.
Substance Name: [TRIS Acetate EDTA 10X Solution]
10X solution contains:
TRIS-Base: 0.4 M
Acetic Acid: 0.2 M
EDTA: 10 mM
pH (10X Solution (@25°C): 8.2-8.4
Research Products International Corp DIPSO 100 G
Substance Name: 3-(N,N-Bis(2-Hydroxyethyl)amino)-2-hydroxypropane sulfonic acid
CAS Number: 68399-80-4
Molecular Formula: C7H17NO6S
Molecular Weight: 243.3
Chemical Purity: >98%
Soluble in:?Water
Store at:?Room Temperature
Research Products International Corp PIPES Sesquisodium Salt 5 KG
PIPES Sesquisodium Salt, also known as PIPES Na3, is a chemical compound that has significant applications in the life science marketplace. PIPES stands for piperazine-N,N'-bis(2-ethanesulfonic acid), and the sesquisodium salt refers to the trisodium salt form of PIPES.
It is primarily utilized as a buffering agent. Buffers are substances that help maintain a stable pH in biological and biochemical processes, ensuring optimal conditions for various enzymatic reactions and cellular activities.
PIPES Sesquisodium Salt is particularly valuable in biological research, molecular biology, and cell culture applications. It offers excellent buffering capacity in the physiological pH range (6.1 - 7.5), making it suitable for a wide range of experimental conditions.
Research Products International Corp POPSO 100 G
CAS Number: 68189-43-5
Molecular Formula: C10H22N2O8S2
Molecular Weight: 362.4
Hampton Research HR2-785/1.0 M HEPES pH 7.0 - 100 ml
HR2-785/1.0 M HEPES pH 7.0 - 100 ml. Please note items from this supplier are non-returnable.
Kd Medical Inc PBS 10X, PH 7.4
Standard buffer for many biomolecular procedures. One 1 Liter bottle.
Kd Medical Inc TBE 10X 1 LITER BOTTLE
Tris Borate EDTA is used for polyacrylamide and agarose gel electrophoresis. 10X TBE is concentrated and ready to be diluted to a 1X working solution. One 1 liter bottle.
Hampton Research HR2-729/1.0 M HEPES pH 7.5 - 100 ml
HR2-729/1.0 M HEPES pH 7.5 - 100 ml. Please note items from this supplier are non-returnable.
Growcells PBS 10X 1L EA
Sterile 10X Phosphate-Buffered Saline solution concentrate prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: NaCl 80g, KCl 2g, Na2HPO4 14.4g, KH2PO4 2.4g
Rockland Immunochemicals 1.0 M HEPES pH 7.2 - MB-062-0100
HEPES is a useful buffer for pH's ranging from 6.8-8.2. This makes HEPES ideal for cell culturing because of its usefulness in maintaining pH changes in presence of carbon dioxide production from cellular respiration.1.0 M HEPES pH 7.2 is ideal for investigators in Molecular Biology, Cell Biology, and Immunology researchers.